3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H2O => (24R, 25R) 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA (THCA-CoA) is hydrated to (24R, 25R) 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA (THCA-CoA) is hydrated to form (24R, 25R) 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA. This reaction, catalyzed by the peroxisomal D-bifunctional enzyme (Huyghe et al. 2006), occurs in the peroxisomal matrix. Authored: Jassal, B, 2007-01-19 10:34:59 EC Number: 4.2.1.107 Edited: D'Eustachio, P, 2007-02-17 19:40:28 Pubmed16766224 Pubmed8902629 Reactome Database ID Release 43192331 Reactome, http://www.reactome.org ReactomeREACT_10019 Reviewed: D'Eustachio, P, 2007-04-30 14:43:26