25(S) DHCA-CoA + O2 => 25(S) 3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA + H2O2 25(S) DHCA-CoA and O2 react to form 25(S) 3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA and H2O2. This dehydrogenation reaction occurs in the peroxisomal matrix. It is catalyzed by the FAD-containing peroxisomal enzyme branched chain acyl-CoA oxidase (ACOX2). The enzyme transfers electrons to molecular oxygen and hydrogen peroxide is produced as a byproduct. 25(S) DHCA-CoA is dehydrogenated to 25(S) 3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA Authored: Jassal, B, 2007-01-19 10:34:59 EC Number: 1.3.3.6 Edited: D'Eustachio, P, 2007-02-17 19:40:28 Pubmed8387517 Pubmed8943006 Reactome Database ID Release 43193369 Reactome, http://www.reactome.org ReactomeREACT_10003 Reviewed: D'Eustachio, P, 2007-04-30 14:43:26