SRC phosphorylates SPRY2 on Y55 and Y227 Authored: Rothfels, K, 2011-08-15 EC Number: 2.7.10 Pubmed15004239 Pubmed15564375 Pubmed15637081 Reactome Database ID Release 431295609 Reactome, http://www.reactome.org ReactomeREACT_111096 Reviewed: Gotoh, N, 2011-08-26 Sprouty 2 protein is phosphorylated on tyrosine residue 55. The ability of SRC kinase to catalyze this reaction has been demonstrated with purified proteins in vitro (Li et al. 2004) and in cultured cells with studies of the effects of SRC-family pharmacological inhibitors and of dominant-negative mutant SRC proteins (Mason et al. 2004). SRC kinase also phosphorylates numerous tyrosine residues in the C terminal region of SPRY2 including Y227, in response to FGF but not EGF stimulation. has a Stoichiometric coefficient of 2 PP2A(A:C):SPRY2 PP2A(A:C):Y55/Y227-pSPRY2