IRAK1:p-S,2T-IRAK4 :oligo-MyD88:Mal:activated TLR p-IRAK1:p-IRAK4:oligo-MyD88:Mal:activated TLR First phosphorylation of IRAK1 by IRAK4 bound to activated TLR:MyD88:Mal Authored: de Bono, B, 2005-08-16 10:54:15 EC Number: 2.7.11 Edited: Shamovsky, V, 2012-11-06 First, IRAK1 is phosphorylated at Thr209 by IRAK4. This results in a conformational change of the kinase domain, permitting further phosphorylations to take place. Substitution of Thr209 by alanine results in a kinase-inactive IRAK1. Pubmed11960013 Pubmed14625308 Reactome Database ID Release 43166119 Reactome, http://www.reactome.org ReactomeREACT_6833 Reviewed: Gay, NJ, 2006-04-24 16:48:17 Reviewed: Gillespie, ME, 2010-11-30 Reviewed: Napetschnig, Johanna, 2012-11-16 has a Stoichiometric coefficient of 4