K63-polyubiquitin K63-linked poly-Ub-IRF7:TRAF6:p-3S,3T-IRAK1:p-S,2T-IRAK4:oligo-MyD88:activated TLR7/8 or 9. IRF7:TRAF6:p-3S,3T-IRAK1:p-S,2T-IRAK4:oligo-MyD88:activated TLR7/8 or 9 TRIKA1 TRAF6 ubiquitinqtes IRF7 in a K63-dependent manner following TLR7/8 or 9 stimulation Authored: Shamovsky, V, 2010-08-25 EC Number: 6.3.2.19 Edited: Shamovsky, V, 2010-11-15 Pubmed15361868 Pubmed18710948 Reactome Database ID Release 43975118 Reactome, http://www.reactome.org ReactomeREACT_25090 Reviewed: Gillespie, ME, 2010-10-29 TRAF6 E3 ubiquitin ligase activity was shown to be essential for IRF7 activation, although the role of TRAF6-dependent ubiquitination remains unclear [Kawai T et al 2004]. It has been demonstrated that IRF7 is ubiquitinated by TRAF6 at multiple sites both in vitro and in vivo[Ning et al 2008].It has been also shown that K63-linked ubiquitination of IRF7 is independent of its C-terminal functional phosphorylation sites. has a Stoichiometric coefficient of 4