RIP1:TRIF:activated TLR3/TLR4 activated TLR3/TLR4:TRIF:RIP1:FADD:pro-caspase-8 TLR3/4-induced ripoptosome assembly Authored: Shamovsky, V, 2012-05-15 Edited: Shamovsky, V, 2012-11-19 Pubmed12181749 Pubmed12752666 Pubmed14739303 Pubmed15814722 Pubmed21737329 Pubmed21737330 Pubmed22421964 Reactome Database ID Release 432562541 Reactome, http://www.reactome.org ReactomeREACT_150447 Reviewed: Fitzgerald, Katherine A, 2012-11-13 TRIF was repored to efficiently induce apoptosis when overexpressed in human HEK293T cells. TRIF-induced apoptosis occurred through activation of the FADD-caspase-8 axis [Kaiser WJ and Offermann MK 2005; Kalai M et al 2002; Estornes Y et al 2012]. C-terminus of TRIF was shown to form complexes with both RIP1 and RIP3, and disruption of these interactions by mutating the RHIM eliminated the ability of TRIF to induce apoptosis [Kaiser WJ and Offermann MK 2005].<p> Prevention of RIP1 ubiquitination leads to a strong association of RIP1 and caspase-8 [Feoktistova M et al 2011, Tenev et al 2011]. has a Stoichiometric coefficient of 2