Autophosphorylation of insulin receptor Authored: Bevan, AP, 2003-07-31 08:01:55 EC Number: 2.7.10.1 For the receptor to autophosphorylate requires a lysine at position 1030 to stabilize the gamma phosphate of ATP whilst the adenosine of ATP itself interacts with three glycines at residues 1003 - 1008. The first tyrosine residues to be autophosphorylated are 1158, 1162 and 1163 in the tyrosine kinase domain. This is shortly followed by tyrosine 972 in the juxtamembrane domain and tyrosines 1328 and 1330. These tyrosines fall into the three distinct tyrosine phosphorylation domains of the beta-subunit. In total there are 13 potential tyrosines that may be phosphorylated. The receptor phosphorylates itself in a trans rather than cis manner. That is one beta-subunit of the receptor phosphorylates the other rather than itself. Pubmed8039601 Pubmed8276779 Reactome Database ID Release 4374715 Reactome, http://www.reactome.org ReactomeREACT_2211 has a Stoichiometric coefficient of 12 activated insulin receptor insulin:insulin receptor