Nephrin binds NCK Authored: de Bono, B, Garapati, P V, 2008-02-26 12:30:30 Edited: Garapati, P V, 2010-03-01 Nephrin tyrosine phophorylation regulates podocyte cell morphology via NCK adaptor proteins. NCK via its SH2 domain interacts with the three phosphotyrosines (1176, 1193 and 1217) on nephrin, and through its SH3 domain can recruit several other proteins involved in the regulation of the actin cytoskeleton such as N WASP, WIP, ARP2/3 and PAK to the slit diaphragm. The importance of this mechanism is the maintenance of the filtration barrier. When rapid actin polymerization and cytoskeletal reorganization is needed, for example during development or injury and repair, the level of nephrin phosphorylation increases and leads to recruitment of NCK and its downstream effectors to the cytoplasmic side of the slit diaphragm. Pubmed16525419 Pubmed16630808 Pubmed19443634 Reactome Database ID Release 43373724 Reactome, http://www.reactome.org ReactomeREACT_23852 Reviewed: Huber, TB, Grahammer, Florian, 2010--0-5- NCK NEPH1:p-Nephrin:Fyn NEPH1:p-Nephrin:Fyn:NCK