PI3K p85 associates with both p-Nephrin and CD2AP Authored: de Bono, B, Garapati, P V, 2008-02-26 12:30:30 Edited: Garapati, P V, 2010-03-01 Reactome Database ID Release 43451758 Reactome, http://www.reactome.org ReactomeREACT_24013 Reviewed: Huber, TB, Grahammer, Florian, 2010--0-5- The regulatory p85 subunit of PI3K recognizes and binds to both phosphorylated nephrin and its binding partner, CD2AP. By mutation analysis, nephrin Y1158 was shown to be necessary for the interaction. This interaction allows the catalytic subunit p110 to act on phospholipids of the inner leaflet of the cell membrane. This leads to downstream phosphorylation and inactivation of the apoptotic factor Bad via the serine-threonine kinase AKT. PI3K:CD2AP:p-Nephrin:Fyn:NEPH1 CD2AP:p-Nephrin:Fyn:NEPH1