Autocleavage of Separase After APC/C-mediated degradation of PTTG1 (securin), ESPL1 (separin i.e. separase) is rapidly autocatalytically cleaved after arginine residues at positions 1506 and 1535. The N-terminal and C-terminal fragments remain bound to each other after cleavage. It has not been examined what happens with the short middle fragment of ESPL1, so it is annotated as a part of the autocleaved ESPL1 complex. The autocatalytic cleavage of ESPL1 is not a prerequisite for the subsequent cleavage of the cohesin subunit RAD21 (Waizenegger et al. 2002). Authored: Orlic-Milacic, M, 2012-10-02 Autocleavage of ESPL1 (Separin) Edited: Gillespie, ME, 2012-10-05 Edited: Matthews, L, 2012-10-05 Pubmed12194817 Reactome Database ID Release 432467775 Reactome, http://www.reactome.org ReactomeREACT_150308 Reviewed: Zhang, Nenggang, 2012-10-22 ESPL1 Autocleaved