PP2A-B56 dephosphorylates centromeric cohesin Authored: Orlic-Milacic, M, 2012-10-02 Edited: Gillespie, ME, 2012-10-05 Edited: Matthews, L, 2012-10-05 PLK1-mediated phosphorylation of the STAG2 subunit of centromeric cohesin (Hauf et al. 2005) is counteracted by the kinetochore PP2A phosphatase, containing the 56 kDa regulatory B subunit (PP2A-B56 i.e. PP2A-PPP2R5). PP2A-B56 is recruited to the centromeric cohesin complex by shugoshin proteins (SGOL1 and SGOL2) (Kitajima et al. 2006), which are also kinetochore constituents (Cheeseman and Desai 2008). SGOL1 localization to centromeres depends on another kinetochore protein, BUB1 (Kitajima et al. 2004, Kitajima et al. 2005). Shugoshin- and PP2A-B56-regulated dephosphorylation of centromeric STAG2 ensures that the cohesin complex remains bound to centromeres throughout prometaphase and metaphase, thereby preventing premature separation of sister chromatids (Salic et al. 2004, Kitajima et al. 2004, Kitajima et al. 2005, Kitajima et al. 2006). Pubmed14730319 Pubmed15339662 Pubmed15723797 Pubmed15737063 Pubmed16541025 Pubmed18097444 Reactome Database ID Release 431638821 Reactome, http://www.reactome.org ReactomeREACT_150250 Reviewed: Zhang, Nenggang, 2012-10-22 p-STAG2,RAD21-Ac-Cohesin:PDS5:p-CDCA5:WAPAL:Sister Centromeres:Kinetochores:Microtubules p-RAD21-Ac-Cohesin:PDS5:p-CDCA5:WAPAL:Sister Centromeres:Kinetochores:Microtubules