Elongation of Extended Telomeric Chromosome End Authored: Blackburn, EH, Seidel, J, 2006-03-09 19:41:08 GENE ONTOLOGYGO:0007004 Pubmed10825187 Pubmed14630939 Pubmed15189140 Pubmed15776019 Pubmed8330740 Pubmed9049315 Pubmed9548747 Reactome Database ID Release 43164617 Reactome, http://www.reactome.org ReactomeREACT_7985 Reviewed: Price, C, 2006-07-13 18:33:38 The elongation reaction proceeds as follows: The template of hTERC directs the sequential addition of nucleotides to the 3' telomeric DNA end. Following addition of a nucleotide, the template and catalytic site must move relative to one another within the telomerase RNP to place the appropriate template residue in the active site. As base-pairing and nucleotide addition occur at one end of the template, base pair melting occurs at the other (Collins and Greider 1993; Wang and Blackburn, 1997; Hammond and Cech 1998; Benjamin et al. 2000; Forstemann and Lingner 2005). This un-pairing is thought to reduce the energy used for mediating the subsequent translocation step. Nucleotide addition can occur up until the template boundary which in hTERC is defined by a helix called P1b (Chen and Greider 2003).<br> Telomerase RNP:Telomeric Chromosome End with an Additional single Stranded Telomere repeat Telomerase Holoenzyme:Telomeric RNP End with Two Additional Single Stranded Telomere Repeats