PathwayStep1315 PathwayStep1317 PathwayStep1316 PathwayStep1318 (S)-Hydroxyoctanoyl-CoA+NAD<=>3-Oxooctanoyl-CoA+NADH+H 3-Oxooctanoyl-CoA+CoA-SH<=>Hexanoyl-CoA trans-Oct-2-enoyl-CoA+H2O<=>(S)-Hydroxyoctanoyl-CoA Octanoyl-CoA+FAD<=>trans-Oct-2-enoyl-CoA+FADH2 Beta oxidation of octanoyl-CoA to hexanoyl-CoA Authored: Gillespie, ME, 2003-09-19 00:00:00 Edited: Gillespie, ME, 0000-00-00 00:00:00 ISBN0079130356 Reactome Database ID Release 4377348 Reactome, http://www.reactome.org ReactomeREACT_1697 The fifth pass through the beta-oxidation spiral picks up where the last left off with the saturated fatty acid octanoyl-CoA and produces hexanoyl-CoA. Four enzymatic steps are required starting with MCAD CoA dehydrogenase (Medium Chain) activity, followed by the enoyl-CoA hydratase activity of crotonase, the 3-hydroxyacyl-CoA dehydrogenase activity of the short chain 3-hydroxyacyl-CoA dehydrogenase (SCHAD), and completed by the ketoacyl-CoA thiolase activity, present in the mitochondrial membrane associated trifunctional protein.