PathwayStep1311 PathwayStep1310 PathwayStep1313 PathwayStep1312 3-Oxodecanoyl-CoA+CoA-SH<=>Octanoyl-CoA (S)-Hydroxydecanoyl-CoA+NAD<=>3-Oxodecanoyl-CoA+NADH+H Decanoyl-CoA+FAD<=>trans-Dec-2-enoyl-CoA+FADH2 trans-Dec-2-enoyl-CoA+H2O<=>(S)-Hydroxydecanoyl-CoA Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA Authored: Gillespie, ME, 2003-09-19 00:00:00 Edited: Gillespie, ME, 0000-00-00 00:00:00 ISBN0079130356 Reactome Database ID Release 4377346 Reactome, http://www.reactome.org ReactomeREACT_1708 The fourth pass through the beta-oxidation spiral picks up where the last left off with the saturated fatty acid decanoyl-CoA and produces octanoyl-CoA. Four enzymatic steps are required starting with MCAD CoA dehydrogenase (Medium Chain) activity, followed by the enoyl-CoA hydratase activity of crotonase, the 3-hydroxyacyl-CoA dehydrogenase activity of the short chain 3-hydroxyacyl-CoA dehydrogenase (SCHAD), and completed by the ketoacyl-CoA thiolase activity, present in the mitochondrial membrane associated trifunctional protein. Note that the 3-hydroxyacyl-CoA dehydrogenase activity of SCHAD is not actually limited to short chain fatty acids, in fact SCHAD has a broad substrate specificity.