PathwayStep1307 PathwayStep1308 PathwayStep1305 PathwayStep1306 lauroyl-CoA+FAD<=>2-trans-Dodecenoyl-CoA+FADH2 2-trans-Dodecenoyl-CoA+H2O<=>(S)-3-Hydroxydodecanoyl-CoA (S)-3-Hydroxydodecanoyl-CoA+NAD<=>3-Oxododecanoyl-CoA+NADH+H 3-Oxododecanoyl-CoA+CoA-SH<=>Decanoyl-CoA Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA Authored: Gillespie, ME, 2003-09-19 00:00:00 Edited: Gillespie, ME, 0000-00-00 00:00:00 ISBN0079130356 Reactome Database ID Release 4377310 Reactome, http://www.reactome.org ReactomeREACT_735 The third pass through the beta-oxidation spiral picks up where the last left off with the saturated fatty acid lauroyl-CoA and produces decanoyl-CoA. Four enzymatic steps are required starting with LCAD CoA dehydrogenase (Long Chain) activity, followed by the enoyl-CoA hydratase activity of crotonase, the 3-hydroxyacyl-CoA dehydrogenase activity of the short chain 3-hydroxyacyl-CoA dehydrogenase (SCHAD), and completed by the ketoacyl-CoA thiolase activity, present in the mitochondrial membrane associated trifunctional protein. Note that the 3-hydroxyacyl-CoA dehydrogenase activity of SCHAD is not actually limited to short chain fatty acids, in fact SCHAD has a broad substrate specificity.