PathwayStep1295 PathwayStep1296 PathwayStep1297 PathwayStep1298 palmitoyl-CoA+FAD<=>trans-Hexadec-2-enoyl-CoA+FADH2 (S)-3-Hydroxyhexadecanoyl-CoA+NAD<=>3-Oxopalmitoyl-CoA+NADH+H trans-Hexadec-2-enoyl-CoA+H2O<=>(S)-3-Hydroxyhexadecanoyl-CoA 3-Oxopalmitoyl-CoA+CoA-SH<=>myristoyl-CoA Beta oxidation of palmitoyl-CoA to myristoyl-CoA Authored: Gillespie, ME, 2003-09-19 00:00:00 Edited: Gillespie, ME, 0000-00-00 00:00:00 ISBN0079130356 Reactome Database ID Release 4377305 Reactome, http://www.reactome.org ReactomeREACT_2025 This first pass through the beta-oxidation spiral starts with the saturated fatty acid palmitoyl-CoA and produces myristoyl-CoA. Four enzymatic steps are required, starting with VLCAD CoA dehydrogenase (Very Long Chain) activity, followed by three enzymatic steps, enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and ketoacyl-CoA thiolase activities, all present in the mitochondrial membrane associated trifunctional protein.