Integrin alphaIIb beta3:p(Y530)-SRC:CSK Talin:RIAM complex:ECM ligands: 2X(Integrin alphaIIb beta3:p(Y530)-SRC:CSK) Focal complex Clustering of Integrin alphaIIb beta3 complexes Authored: Garapati, P V, 2008-06-16 17:13:06 Edited: Garapati, P V, 2008-06-16 17:13:06 Pubmed10508650 Pubmed15157149 Pubmed16005629 Reactome Database ID Release 43377641 Reactome, http://www.reactome.org ReactomeREACT_15403 Reviewed: Shattil, SJ, 2008-09-16 06:21:39 The fibrinogen-bound integrin alphaIIb beta3 clusters platelets together to form a platelet plug and generates intracellular signals (outside-in) causing further platelet activation and platelet plug retraction.<br>Intracellular integrin alphaIIb beta3 clustering brings SRCs bound to integrin beta3 chains into proximity. SRC associates constitutively with integrin alphaIIb beta3. In unstimulated cells this SRC is inactive, auto-inhibited by an internal interaction between phosphorylated Y530 and the SH2 domain. CSK is selective for the Y530 residue and prevents access to SRC of PTP1B, a protein tyrosine phosphatase that is capable of de-phosphorylating Y530.