Talin:RIAM complex Rap1-GTP:PIP2:RIAM Activation of Talin Authored: Garapati, P V, 2008-06-16 17:13:06 Edited: Garapati, P V, 2008-06-16 17:13:06 Pubmed12585966 Pubmed16102045 Pubmed17624957 Pubmed18434644 Reactome Database ID Release 43354097 Reactome, http://www.reactome.org ReactomeREACT_15350 Reviewed: Shattil, SJ, 2008-09-16 06:21:39 Talin is one of the major cytoskeletal proteins involved in integrin activation and linking the resulting focal adhesion (FA) with cytoskeleton. Talin comprises an N-ter head region and a flexible rod domain. The head region has the FERM domain (subdivided into F1, F2 and F3 subdomains), which has the binding sites for beta integrin cytoplasmic regions and actin binding sites close to the C-terminal rod domain. <br>Talin exists in closed inactive conformation, where the head region interacts with the rod domain masking the integrin binding sites. At the plasma membrane the RIAM bound to active Rap1 recruits talin to form the integrin activation complex. This interaction exposes the integrin-binding site in talin F3 domain leading to integrin activation. <br>