Grasp65:Gm130:p115:RAB1:GTP Uso1 homodimer p-S217,277,376-Grasp65:p-S37-Gm130:p-RAB1:GTP Phosphorylation of Gorasp1, Golga2 and RAB1A by CDK1:CCNB Authored: Orlic-Milacic, M, 2012-07-20 EC Number: 2.7.11.22 Edited: Gillespie, ME, 2012-08-07 Pubmed10679020 Pubmed11238451 Pubmed11285137 Pubmed11306556 Pubmed15678101 Pubmed1902553 Pubmed7737117 Pubmed9753325 Reactome Database ID Release 432422970 Reactome, http://www.reactome.org ReactomeREACT_147793 Reviewed: Colanzi, Antonino, 2012-08-21 Reviewed: Wang, Yanzhuang, 2012-08-19 The existence of Gorasp1:Golga2:Uso1:Rab1:GTP complex was established by co-immunoprecipitation of exogenously expressed human RAB1A (identical sequence to rat Rab1A) from normal rat kidney cells, NRKs (Moyer et al. 2001). It was also shown that human RAB1B binds human GM130 (Weide et al. 2001). In mitotic prophase, human CDK1 (CDC2) in complex with either CCNB1 (cyclin B1) or CCNB2 (cyclin B2), as both CCNB1 and CCNB2 can localize to Golgi (Jackman et al. 1995, Draviam et al. 2001), was shown to phosphorylate recombinant rat Gorasp1 and Golga2, as well as RAB1A (Bailly et al. 1991, Lowe et al. 1998, Preisinger et al. 2005) . Phosphorylation of Golga2 and RAB1A impairs their association with Uso1 (Lowe et al. 1998, Seeman et al. 2000, Moyer et al. 2001). has a Stoichiometric coefficient of 5