PathwayStep1966 PathwayStep1969 PathwayStep1968 PathwayStep1967 PathwayStep1988 PathwayStep1987 PathwayStep1986 PathwayStep1985 PathwayStep1984 PathwayStep1983 PathwayStep1982 PathwayStep1981 PathwayStep1989 PathwayStep1990 PathwayStep1975 PathwayStep1974 PathwayStep1977 PathwayStep1976 PathwayStep1971 PathwayStep1970 PathwayStep1973 PathwayStep1972 PathwayStep1979 PathwayStep1978 PathwayStep1980 “CERT” dissociates from the endoplasmic reticulum CERT [ER] + ceramide [ER] => CERT:ceramide [ER] CERT + ATP => monophospho-CERT + ADP 3-ketosphinganine + NADPH + H+ => sphinganine + NADP+ palmitoyl-CoA + serine => 3-ketosphinganine + CoASH + CO2 multiphospho-CERT:PPM1L:VAPA/B + 3 H2O => CERT:PPM1L:VAPA/B + 3 orthophosphate phosphatidylcholine + ceramide <=> sphingomyelin + diacylglycerol [SGMS1] dihydroceramide + NADPH + H+ + O2 => phytoceramide + NADP+ + H2O dihydroceramide + NAD(P)H + H+ + O2 => ceramide + NAD(P)+ + H2O multiphospho-CERT + PPM1L:VAPA/B => multiphospho-CERT:PPM1L:VAPA/B monophospho-CERT + 2 ATP => multiphospho-CERT + 2 ADP CERT:ceramide [ER] => ceramide [Golgi] + CERT [ER] ceramide + H2O <=> stearate + sphingosine [endoplasmic reticulum] ceramide + H2O => stearate + sphingosine [Golgi] phytoceramide + H2O => stearate + phytosphingosine phosphatidylcholine + ceramide <=> sphingomyelin + diacylglycerol [SGMS2] sphinganine + stearyl-CoA => dihydroceramide + CoASH sphinganine (dihydrosphingosine) +ATP => sphinganine 1-phosphate + ADP sphinganine 1-phosphate + H2O => sphinganine + orthophosphate sphinganine 1-phosphate => phosphoethanolamine + hexadecanal sphingosine +ATP => sphingosine 1-phosphate + ADP sphingosine 1-phosphate + H2O => sphingosine + orthophosphate [cytosolic - PPAP] sphingosine 1-phosphate + H2O => sphingosine + orthophosphate [cytosolic - SGPP] sphingosine 1-phosphate + H2O => sphingosine + orthophosphate [extracellular] sphingosine 1-phosphate => phosphoethanolamine + hexadec-2-enal Sphingolipid de novo biosynthesis Authored: D'Eustachio, P, 2009-08-20 Edited: Jassal, B, 2011-10-14 GENE ONTOLOGYGO:0030148 Pubmed12011104 Pubmed20919643 Reactome Database ID Release 431660661 Reactome, http://www.reactome.org ReactomeREACT_115810 The main steps involved in de novo sphingolipid synthesis are annotated here (Merrill 2002, Gault et al. 2010).