80S:Met-tRNAi:mRNA eIF5B:GDP 80S:Met-tRNAi:mRNA:eIF5B:GTP eIF5B:GTP is hydrolyzed and released Once the 60S subunit joins the translation initiation complex, eIF5B hydrolyzes its GTP and is released from the now 80S monosome. The fully assembled 80s ribosome is now ready to start elongation of the polypeptide chain. Pubmed10659855 Pubmed1095581 Reactome Database ID Release 4372671 Reactome, http://www.reactome.org ReactomeREACT_3