eIF1 eIF4A subunits complex eIF3 subunits complex 48S complex 40S:Met-tRNAi:mRNA eIF2:GDP eIF2:GTP is hydrolyzed, eIFs are released Once the Met-tRNAi has recognized the AUG, eIF2-bound GTP is hydrolyzed. The reaction is catalyzed by eIF5 (or eIF5B) and is thought to cause dissociation of all other initiation factors and allow joining of the large 60S ribosomal subunit. Release of the initiation factors from 40S leaves the Met-tRNAi in the ribosomal P-site base-paired to the start codon on the mRNA. Pubmed10659855 Pubmed1095581 Pubmed11018020 Pubmed1856230 Pubmed429297 Pubmed592398 Pubmed592399 Pubmed641056 Reactome Database ID Release 4372619 Reactome, http://www.reactome.org ReactomeREACT_1060