80S:Met-tRNAi:mRNA 80S:aminoacyl tRNA:mRNA:eEF1A:GTP eEF1A:GTP:aminoacyl-tRNA complex Aminoacyl-tRNA binds to the ribosome at the A-site Authored: Gopinathrao, G, 2005-03-11 20:46:56 Once the correct codon-anticodon match occurs between the mRNA and aa-tRNA, the decoding event triggers GTP hydrolysis on eEF1A. The resulting conformational change releases the aa-tRNA to the A-site, and GDP bound form eEF1A is released from the ribosome.<br>Insight into the mechanics of this system has been obtained from earlier works with rabbit reticulocytes and the E.coli system.<br>This process is illustrated below with: an amino acyl-tRNA with an amino acid, a peptidyl-tRNA with a growing peptide and a ribosome with A,P and E sites to accommodate these two forms of tRNA. Pubmed8722040 Reactome Database ID Release 43156907 Reactome, http://www.reactome.org ReactomeREACT_2075