eEF2:GTP elongating polypeptide chain eEF2:GDP eEF1A:GDP Elongation complex with growing peptide chain eEF1A:GTP:aminoacyl-tRNA complex Translocation of ribosome by 3 bases in the 3' direction Authored: Gopinathrao, G, 2005-02-08 19:10:56 Following peptide bond formation, GTP-bound eEF2 catalyzes the translocation of the deacylated tRNA in the P-site and the peptidyl-tRNA in the A-site (the pre-translocation state) into the E- and P- sites (the post-translocation state), respectively. Thus, the mRNA advances by three bases to expose the next codon in the A-site. After translocation, GDP-bound eEF2 leaves the ribosome to allow another round of elongation. eEF2 is reactivated by the release of GDP and binds GTP for subsequent rounds. <br>This process is illustrated below with a peptidyl-tRNA with a growing peptide, a deacylated tRNA with an -OH and a ribosome with A,P and E sites to accommodate these three forms of tRNA is also shown. Pubmed1620067 Pubmed1703007 Reactome Database ID Release 43156915 Reactome, http://www.reactome.org ReactomeREACT_1937 has a Stoichiometric coefficient of 2