PathwayStep581 PathwayStep580 PathwayStep582 PathwayStep579 PathwayStep577 PathwayStep578 PathwayStep550 ADP binds to P2Y purinoceptor 1 Src activation following P2Y purinoceptor activation P2Y purinoceptor 1 activates MAP kinase p38 alpha Gq activation by P2Y purinoceptor 1 Dissociation of the P2Y purinoceptor 1:Gq complex Activated P2Y purinoceptor 1 binds G-protein Gq Phosphorylation of cPLA2 by MAPK p38 alpha ADP signalling through P2Y purinoceptor 1 Authored: Jupe, S, 2009-04-24 10:43:00 Co-activation of P2Y1 and P2Y12 is necessary for complete platelet activation. P2Y1 is coupled to Gq and helps trigger the release of calcium from internal stores, leading to weak and reversible platelet aggregation. P2Y12 is Gi coupled, inhibiting adenylate cyclase, leading to decreased cAMP, a consequent decrease in cAMP-dependent protein kinase activity which increases cytoplasmic [Ca2+], necessary for activation (Woulfe et al. 2001).<br> In activated platelets, P2Y12 signaling is required for the amplification of aggregation induced by all platelet agonists including collagen, thrombin, thromboxane, adrenaline and serotonin. P2Y12 activation causes potentiation of thromboxane generation, secretion leading to irreversible platelet aggregation and thrombus stabilization. Edited: Jupe, S, 2009-09-10 Pubmed11413156 Pubmed16466948 Pubmed9442040 Reactome Database ID Release 43418592 Reactome, http://www.reactome.org ReactomeREACT_19140 Reviewed: Akkerman, JW, 2009-09-04