PathwayStep490 PathwayStep491 PathwayStep493 PathwayStep492 Formation of Processive Complex RFC dissociates after sliding clamp formation Loading of PCNA - Sliding Clamp Formation RFC binding displaces Pol Alpha Polymerase switching After the primers are synthesized, Replication Factor C binds to the 3'-end of the initiator DNA to trigger polymerase switching. The non-processive nature of pol alpha catalytic activity and the tight binding of Replication Factor C to the primer-template junction presumably lead to the turnover of the pol alpha:primase complex. After the Pol alpha-primase primase complex is displaced from the primer, the proliferating cell nuclear antigen (PCNA) binds to form a "sliding clamp" structure. Replication Factor C then dissociates, and DNA polymerase delta binds and catalyzes the processive synthesis of DNA. Pubmed1670772 Pubmed1671046 Pubmed1967833 Reactome Database ID Release 4369091 Reactome, http://www.reactome.org ReactomeREACT_1792