TNRC6 (GW182) RNA (exact match) Nonendonucleolytic RISC:Target RNA (exact match) Argonaute1/3/4: Guide RNA Binding of Exactly Matching Target RNAs by Nonendonucleolytic RISC Authored: May, B, 2009-06-10 Edited: May, B, 2009-06-10 Pubmed16284623 Pubmed16289642 Pubmed17128271 Pubmed17632058 RISCs containing Argonautes1, 3, and 4 (AGO1/3/4) bind to target RNAs by base-pairing between the target RNA and the guide RNA of the RISC. AGO1/3/4 do not possess ribonuclease activity therefore exact matches between the guide and the target do not result in cleavage of the target. Rather, the effect of binding is inhibition of translation followed by decay of the target RNA. Argonaute proteins have been shown to interact with ribosomal proteins and with components of processing bodies (P-bodies) where RNA degradation occurs. Direct interaction between AGO and a TNRC6 protein is required for inhibition of translation and targeting to P-bodies in vivo. Reactome Database ID Release 43426522 Reactome, http://www.reactome.org ReactomeREACT_118624 Reviewed: Tomari, Y, 2012-02-10