TNRC6 (GW182) RNA (inexact match) Minimal RISC RISC:Target RNA (inexact match) Binding of Inexactly Matching Target RNAs by RISC. Authored: May, B, 2009-06-10 Edited: May, B, 2009-06-10 Pubmed15014042 Pubmed16284623 Pubmed16289642 Pubmed17128271 Pubmed17632058 Pubmed18461144 Pubmed18978028 Pubmed19383768 Pubmed19398495 Pubmed19470757 RISCs can bind target RNAs that do not exactly match the guide RNA carried by Argonaute. Binding is especially dependent on base-pairing between the target RNA and the eight 5' nucleotides of the guide RNA (miRNA or siRNA). After binding, Argonautes1,3, and 4 are incapable of cleavage in all cases. Argonaute2 is capable of cleaving the target RNA but not if mismatches exist in the middle of the guide (centered about 10 nucloetides from the 5' end of the guide RNA). In the absence of cleavage the target RNA remains bound by the RISC, which inhibits translation of the target RNA and causes the RNA to enter the decay pathway. In vivo, inhibition of translation requires interaction of AGO with a TNRC6 protein and MOV10. Reactome Database ID Release 43426489 Reactome, http://www.reactome.org ReactomeREACT_118839 Reviewed: Tomari, Y, 2012-02-10