Formation of HIV-1 elongation complex containing HIV-1 Tat Authored: Matthews, L, Rice, AP, 2005-07-27 00:00:00 Edited: Matthews, L, 2005-07-26 23:29:22 Pubmed10757782 Pubmed14701750 Pubmed15564463 Pubmed7853496 Pubmed9491887 Reactome Database ID Release 43167200 Reactome, http://www.reactome.org ReactomeREACT_6346 This HIV-1 event was inferred from the corresponding human RNA Poll II transcription event in Reactome. The details relevant to HIV-1 are described below. For a more detailed description of the general mechanism, see the link to the corresponding RNA Pol II transcription event below. The formation of the HIV-1 elongation complex involves Tat mediated recruitment of P-TEFb(Cyclin T1:Cdk9) to the TAR sequence (Wei et al, 1998) and P-TEFb(Cyclin T1:Cdk9) mediated phosphorylation of the RNA Pol II CTD as well as the negative transcriptional elongation factors DSIF and NELF (Herrmann, 1995; Ivanov et al. 2000; Fujinaga et al. 2004; Zhou et al., 2004). PathwayStep3567 PathwayStep3565 PathwayStep3566 PathwayStep3564 PathwayStep3563 Hyperphosphorylation (Ser2) of RNA Pol II CTD by the P-TEFb(Cyclin T1:Cdk9) complex Association of Tat with P-TEFb(Cyclin T1:Cdk9) Phosphorylation of NEFL by the P-TEFb(Cyclin T1:Cdk9) complex Phosphorylation of DSIF by the P-TEFb(Cyclin T1:Cdk9) complex Recruitment of elongation factors to form HIV-1 elongation complex