Abortive elongation of HIV-1 transcript in the absence of Tat Pubmed10199401 Pubmed2825027 Pubmed9450929 Reactome Database ID Release 43167242 Reactome, http://www.reactome.org ReactomeREACT_6261 This event was inferred from the corresponding Reactome human Poll II transcription elongation event. The details specific to HIV-1 transcription elongation are described below. In the absence of the HIV-1 Tat protein, the RNA Pol II complexes associated with the HIV-1 template are non-processive. RNA Pol II is arrested after promoter clearance by the negative transcriptional elongation factors DSIF and NELF as occurs during early elongation of endogenous templates (Wada et al, 1998; Yamaguchi et al. 1999). This arrest cannot be overcome by P-TEFb mediated phosphorylation in the absence of Tat however, and elongation aborts resulting in the accumulation of short transcripts (Kao et al., 1987). PathwayStep3574 PathwayStep3573 Separation of abortive HIV-1 transcript from template Limited elongation of the HIV-1 transcript