Membrane binding and targetting of GAG proteins GENE ONTOLOGYGO:0046788 ISBN0-87969-571-4 One of the mysteries of Gag protein involvement in HIV virion assembly is how the proteins are targeted to the proper membrane for budding. Infectious retroviruses do not bud from all of the available membrane surfaces within an infected cell, but primarily from the plasma membrane, which constitutes a small proportion of the total membrane surface in most cells. In polarized cells, the sites of budding are further restricted to the basolateral membrane. Pubmed15473846 Pubmed16683918 Reactome Database ID Release 43174490 Reactome, http://www.reactome.org ReactomeREACT_115893 PathwayStep3486 PathwayStep3487 PathwayStep3488 N-myristoylation of GAG polyprotein by NMT2 Monoubiquitination of N-myristoyl GAG polyprotein Monoubiquitinated N-myristoyl GAG polyprotein associates with an ESCRT-I complex at a late endosomal membrane