vRNP Assembly Authored: Bortz, E, Garcia-Sastre, A, 2007-02-12 19:39:05 For each of eight gene segments, a viral ribonucleoprotein (vRNP), containing a viral negative-sense RNA (vRNA) segment complexed with nucleoprotein (NP) and the trimeric influenza polymerase (PB1, PB2, and PA), is assembled in the nucleus (Braam, 1983; Jones, 1986; Cros, 2003; reviewed in Buolo, 2006). The vRNP functions in three modes (reviewed in Mikulasova, 2000; Neumann, 2004): (1) transcription, which synthesizes viral messenger RNA from the vRNA template using as primers 5' ends of cellular mRNAs containing the cap; (2) replication, which produces positive-sense complementary RNA (cRNA) and subsequently vRNA, both complexed with NP and the trimeric polymerase; or (3), the vRNP is exported from the nucleus into the cytoplasm and is incorporated into assembling virions at the plasma membrane. Pubmed11252672 Pubmed12921991 Pubmed15298169 Pubmed17081640 Pubmed3023071 Pubmed6616622 Reactome Database ID Release 43192905 Reactome, http://www.reactome.org ReactomeREACT_9434 Reviewed: Squires, B, 2007-02-12 19:39:22 PathwayStep3692 PathwayStep3693 NP binds vRNA Viral Polymerase Assembly