Nef Mediated CD8 Down-regulation Authored: Gillespie, ME, 2007-07-25 19:42:36 GENE ONTOLOGYGO:0050690 Human immunodeficiency virus (HIV) Nef is a membrane-associated protein decreasing surface expression of CD4, CD28, and major histocompatibility complex class I on infected cells. Nef also strongly down-modulates surface expression of the beta-chain of the CD8alphabeta receptor by accelerated endocytosis, while CD8 alpha-chain expression is less affected. Mutational analysis of the cytoplasmic tail of the CD8 beta-chain indicates that an FMK amino acid motif is critical for the Nef-induced endocytosis. Although independent of CD4, endocytosis of the CD8 beta-chain is abrogated by the same mutations in Nef that affect CD4 down-regulation, suggesting common molecular interactions. The ability to down-regulate the human CD8 beta-chain was conserved in HIV-1, HIV-2, and simian immunodeficiency virus SIVmac239 Nef and required an intact AP-2 complex. Pubmed12032142 Pubmed16103193 Reactome Database ID Release 43182218 Reactome, http://www.reactome.org ReactomeREACT_11200 Reviewed: Skowronski, J, 2007-08-07 14:33:41 PathwayStep3664 PathwayStep3663 PathwayStep3662 Degradation of CD8 Formation of CD8:Nef:AP-2 Complex:v-ATPase Complex Internalization of the CD8:Nef:AP-2 Complex:v-ATPase Complex