PathwayStep2677 PathwayStep2678 PathwayStep2679 FMO oxidizes nucleophiles Authored: Jassal, B, 2008-05-19 12:57:01 Edited: Jassal, B, 2008-05-19 12:57:01 Flavin-containing monooxygenases (FMOs) are the second family of microsomal oxidative enzymes with broad and overlapping specificity. The major reactions FMOs catalyze are nucleophilic hetero-atom compounds such as nitrogen, sulfur or phosphorus as the hetero-atom to form N-oxides, S-oxides or P-oxides respectively. Despite the functional overlap with cytochrome P450s, the mechanism of action differs. FMOs bind and activate molecular oxygen before the substrate binds to the enzyme (picture). They also require flavin adenosine dinucleotide (FAD) as a cofactor. Unlike cytochrome P450 enzymes, FMOs are heat-labile, a useful way to distinguish which enzyme system is at work for researchers studying metabolism. Also, FMOs are not inducible by substrates, unlike the P450 enzymes.\n(1) NADPH binds to the enzyme and reduces the prosthetic group FAD to FADH2. NADP+ remains bound to the enzyme.\n(2) Incorporation of molecular oxygen to form a hydroperoxide.\n(3) A peroxide oxygen is transferred to the substrate.\n(4) Water is released.\n(5) NADP+ dissociates returning the enzyme to its initial state.\n\nTo date, there are 6 isozymes of FMO (FMO1-6) in humans, the most prominent and active one being FMO3. The FMO6 gene does not encode for a functional enzyme although it has the greatest sequence similarity with FMO3 (71%), whilst the others range from 50-58% sequence similarity with FMO3. FMO1-3 are the ones that exhibit activity towards nucleophiles, the others are insignificant in this respect. Pubmed12951812 Pubmed15922018 Reactome Database ID Release 43217271 Reactome, http://www.reactome.org ReactomeREACT_13653 Reviewed: D'Eustachio, P, 2008-05-28 08:30:54 FMO3 N-oxidizes the tertiary amine trimethylamine FMO2 S-oxidizes the antithyroid drug methimazole FMO1 N-oxidizes the anti-cancer drug tamoxifen