PathwayStep2912 PathwayStep2914 PathwayStep2913 ABH2 mediated Reversal of Alkylation Damage AlkB is an alpha-ketoglutarate- and Fe(II)-dependent dioxygenase that oxidizes the relevant methyl groups and releases them as formaldehyde. The human homologs of both these enzymes remove 1-methyladenine and 3-methylcytosine from methylated polynucleotides in an alpha-ketoglutarate-dependent reaction. They act by direct damage reversal with the regeneration of the unsubstituted bases. E.coli AlkB, and human ABH2, and ABH3 can also repair 1-ethyladenine residues in DNA with the release of acetaldehyde (Duncan et al., 2002). GENE ONTOLOGYGO:0006307 Pubmed12486230 Pubmed15040447 Pubmed16174769 Reactome Database ID Release 43112122 Reactome, http://www.reactome.org ReactomeREACT_526 Oxidative demethylation of 1-MeA damaged DNA By ABH2 Oxidative demethylation of 1-EtA damaged DNA By ABH2 Oxidative demethylation of 3-MeC damaged DNA By ABH2