PathwayStep2948 PathwayStep2949 PathwayStep2950 Assembly of the RAD51-ssDNA nucleoprotein complex Authored: Matthews, L, 2003-09-10 06:00:00 GENE ONTOLOGYGO:0000730 Pubmed10921897 Pubmed11080452 Pubmed11459989 Pubmed11751636 Pubmed12606939 Reactome Database ID Release 4376000 Reactome, http://www.reactome.org ReactomeREACT_2141 The RAD51-ssDNA nucleoprotein filament is a right-hand helical nucleoprotein filament referred to as the presynaptic filament. This nucleoprotein complex contains a binding site for the double-stranded homologous DNA molecule with which it interacts during strand exchange. Loading of RAD51 onto ssDNA is thought to be facilitated by recombination mediator RAD52 (McIlwraith et al., 2000), RAD51 paralogs: RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3 (Sigurdsson et al., 2001), by the association of RAD52 with ssDNA (Parsons et al., 2000) and by the association of RAD51 with BRCA2 (Tarsounas et al., 2003). Association of RAD51 with RAD52:DNA double-strand break ends Association of RAD51 with the RPA complex Association of RAD51 with the resected ends of the DNA double-strand break