PathwayStep176 PathwayStep190 PathwayStep192 PathwayStep191 RNA Polymerase III Promoter Opening at Type 2 Promoters Binding of TFIIIC to Type 2 promoter Binding of TFIIIB to TFIIC: Type 2 Promoter Complex Recruitment of RNA Polymerase III to the TFIIIB:TFIIIC: Type 2 Promoter Complex RNA Polymerase III Transcription Initiation From Type 2 Promoter Authored: Hernandez, N, 2003-09-11 07:42:29 Edited: Joshi-Tope, G, 0000-00-00 00:00:00 Reactome Database ID Release 4376066 Reactome, http://www.reactome.org ReactomeREACT_1036 The type 2 promoters can recruit TFIIIC without the help of TFIIIA because TFIIIC binds directly to the A and B boxes. As for the type 1 promoters, this then allows the binding of Brf1-TFIIIB and RNA polymerase III. Importantly, in the yeast system, once Brf1-TFIIIB has been recruited to type 1 or 2 promoters, TFIIIA and/or TFIIIC can be stripped from the DNA with high salt or heparin treatment. Brf1-TFIIIB remains bound to the DNA and is sufficient to direct multiple rounds of transcription .