PathwayStep319 PathwayStep318 PathwayStep317 Binding of Lsm1-7 Complex to Deadenylated mRNA 5' to 3' Exoribonuclease Digestion of Decapped mRNA Decapping of mRNA by the DCP1-DCP2 Complex mRNA Decay by 5' to 3' Exoribonuclease Authored: May, B, 2009-07-22 Degradation of mRNA from 5' to 3' occurs in three steps. First, the mRNA is bound at its 3' end by the Lsm1-7 complex. The bound Lsm1-7 may prevent nucleases from accessing the 3' end. Second, the 7-methylguanosine cap of the mRNA is hydrolyzed by the DCP1-DCP2 complex. Third, the 5' end of the decapped mRNA is attacked by the XRN1 exoribonuclease which digests the remainder of the mRNA from 5' to 3'. These processes may be physically connected by PATL1, the homolog of yeast Pat1, which stably binds the Lsm1-7 complex and interacts with the DCP1-DCP2 decapping complex and the Ccr4-NOT deadenylation complex (Ozgur et al. 2010). Edited: May, B, 2009-07-22 GENE ONTOLOGYGO:0043928 Pubmed11283721 Pubmed14749774 Pubmed15475613 Pubmed16141059 Pubmed17245413 Pubmed19239894 Pubmed20584987 Reactome Database ID Release 43430039 Reactome, http://www.reactome.org ReactomeREACT_20518 Reviewed: Wilusz, J, 2009-09-17