PathwayStep285 PathwayStep284 PathwayStep283 PathwayStep268 PathwayStep267 PathwayStep266 PathwayStep265 PathwayStep269 PathwayStep270 PathwayStep271 PathwayStep273 PathwayStep272 PathwayStep275 PathwayStep274 PathwayStep277 PathwayStep276 PathwayStep279 PathwayStep278 PathwayStep280 PathwayStep281 PathwayStep282 alanine + tRNA(Ala) + ATP => Ala-tRNA(Ala) + AMP + pyrophosphate arginine + tRNA(Arg) + ATP => Arg-tRNA(Arg) + AMP + pyrophosphate asparagine + tRNA(Asn) + ATP => Asn-tRNA(Asn) + AMP + pyrophosphate aspartate + tRNA(Asp) + ATP => Asp-tRNA(Asp) + AMP + pyrophosphate cysteine + tRNA(Cys) + ATP => Cys-tRNA(Cys) + AMP + pyrophosphate glutamate + tRNA(Glu) + ATP => Glu-tRNA(Glu) + AMP + pyrophosphate glutamine + tRNA(Gln) + ATP => Gln-tRNA(Gln) + AMP + pyrophosphate glycine + tRNA(Gly) + ATP => Gly-tRNA(Gly) + AMP + pyrophosphate phenylalanine + tRNA(Phe) + ATP => Phe-tRNA(Phe) + AMP + pyrophosphate proline + tRNA(Pro) + ATP => Pro-tRNA(Pro) + AMP + pyrophosphate lysine + tRNA(Lys) + ATP => Lys-tRNA(Lys) + AMP + pyrophosphate methionine + tRNA(Met) + ATP => Met-tRNA(Met) + AMP + pyrophosphate tryptophan + tRNA(Trp) + ATP => Trp-tRNA(Trp) + AMP + pyrophosphate tyrosine + tRNA(Tyr) + ATP =>Tyr-tRNA(Tyr) + AMP + pyrophosphate serine + tRNA(Ser) + ATP => Ser-tRNA(Ser) + AMP + pyrophosphate threonine + tRNA(Thr) + ATP => Thr-tRNA(Thr) + AMP + pyrophosphate leucine + tRNA(Leu) + ATP => Leu-tRNA(Leu) + AMP + pyrophosphate isoleucine + tRNA(Ile) + ATP => Ile-tRNA(Ile) + AMP + pyrophosphate histidine + tRNA(His) + ATP => His-tRNA(His) + AMP + pyrophosphate pyrophosphate + H2O => 2 orthophosphate [cytosolic] valine + tRNA(Val) + ATP => Val-tRNA(Val) + AMP + pyrophosphate Cytosolic tRNA aminoacylation Authored: D'Eustachio, P, 2008-11-29 15:41:00 Cytosolic tRNA synthetases catalyze the reactions of tRNAs encoded in the nuclear genome, their cognate amino acids, and ATP to form aminoacyl-tRNAs, AMP, and pyrophosphate. Eight of the tRNA synthetases, those specific for arginine, aspartate, glutamate and proline, glutamine, isoleucine, leucine, lysine, and methionine, associate to form a complex with three accessory proteins. Each of the component synthetases is active in vitro as a purified protein; complex formation is thought to channel aminoacylated tRNAs more efficiently to the site of protein synthesis in mRNA:ribosome complexes (Quevillon et al. 1999; Wolfe et al. 2003, 2005). Edited: D'Eustachio, P, 2008-11-29 15:41:00 GENE ONTOLOGYGO:0006418 Pubmed14500886 Pubmed16169847 Pubmed9878398 Reactome Database ID Release 43379716 Reactome, http://www.reactome.org ReactomeREACT_15306 Reviewed: Antonellis, A, 2008-12-02 16:58:45