PathwayStep2970 PathwayStep2971 Processing of DNA ends prior to end rejoining Authored: Matthews, L, 2003-09-07 08:47:00 Ionizing radiation induced DNA double strand breaks often contain end groups that must be processed before the DNA can be rejoined. Possible candidate proteins for the processing steps include Artemis (a nuclease) (Ma and Lieber, 2002); WRN (a helicase with exonuclease activity) (Li and Comai, 2001; Yannone et al., 2002; Karmakar, et al., 2002a, 2002b); PNK (a 5'-OH kinase and 3'-phosphate phosphatase) (Chappell et al., 2002); the Mre11/Rad50/Nbs1 nuclease complex (D' Amours and Jackson, 2002); or hTdp1 (tyrosyl-DNA phosphodiesterase, which removes 3'-glycolates)(Inamdar et al., 2002; Valerie and Povirk, 2003). Precisely when processing of the DNA ends occurs during NHEJ is not known. Pubmed11152456 Pubmed11477099 Pubmed11889123 Pubmed11955432 Pubmed11988766 Pubmed12023295 Pubmed12032095 Pubmed12177300 Pubmed12947387 Reactome Database ID Release 4375924 Reactome, http://www.reactome.org ReactomeREACT_1201 Removal of 3'-phosphate moiety from DSB ends Removal of 3'-phosphoglycolate (PG) moiety from DSB ends