PathwayStep2980 PathwayStep2981 PathwayStep2983 PathwayStep2982 Formation of incision complex in GG-NER Authored: Joshi-Tope, G, 2003-07-14 15:01:00 Binding of XPC complex to the damaged site on the DNA substrate is followed by XPA and RPA recruitment. XPA is a metalloprotein that binds to different types of DNA damage. Binding of RPA, or Replicative protein A, a heterotrimer, initiates the formation of incision complex. This includes a serial assembly of the rest of the NER machinery consisting of TFIIH, XPG etc. Action of helicases in TFIIH complex opens up a bubble structure in the DNA template, exposing a fragment of DNA with lesion for endonuclease activity.<BR> Reactome Database ID Release 4373935 Reactome, http://www.reactome.org ReactomeREACT_257 Formation of ERCC1-XPF heterodimeric complex Binding of ERCC1-XPF to preincision complex Recruitment of repair factors to form preincision complex Formation of open bubble structure in DNA by helicases