PathwayStep400 PathwayStep401 PathwayStep399 The 60S subunit joins the translation initiation complex eIF5B:GTP is hydrolyzed and released eIF2:GTP is hydrolyzed, eIFs are released GTP hydrolysis and joining of the 60S ribosomal subunit Hydrolysis of eIF2-GTP occurs after the Met-tRNAi has recognized the AUG. This reaction is catalyzed by eIF5 (or eIF5B) and is thought to cause dissociation of all other initiation factors and allow joining of the large 60S ribosomal subunit. The 60S subunit joins - a reaction catalyzed by eIF5 or eIF5B - resulting in a translation-competent 80S ribosome. Following 60S subunit joining, eIF5B hydrolyzes its GTP and is released from the 80S ribosome, which is now ready to start elongating the polypeptide chain. Pubmed10659855 Pubmed1095581 Pubmed11018020 Pubmed1856230 Pubmed429297 Pubmed592398 Pubmed592399 Pubmed641056 Reactome Database ID Release 4372706 Reactome, http://www.reactome.org ReactomeREACT_2085