PathwayStep394 PathwayStep391 PathwayStep390 PathwayStep388 PathwayStep389 Cap-bound mRNA is activated by helicases Release of eIF4E from the inactive eIF4E:4E-BP complex Formation of the cap-binding eIF4F complex eIF4F binds to mRNP Translation initiation complex formation Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S Pubmed592399 Pubmed641056 Pubmed6853548 Pubmed9732867 Reactome Database ID Release 4372662 Reactome, http://www.reactome.org ReactomeREACT_1258 The cap-binding complex is constituted by the initiation factors eIF4A, eIF4G and eIF4E. First, eIF4E must be released from the inactive eIF4E:4E-BP complex. Then eIF4A interacts with eIF4G, and eIF4E binds to the amino-terminal domain of eIF4G, resulting in the formation of the cap-binding complex eIF4F. eIF4A together with eIF4B or eIF4H is thought to unwind RNA secondary structures near the 5'-end of the mRNA. The translation initiation complex is formed when the 43S complex binds the cap-bound mRNA.