PathwayStep402 PathwayStep405 PathwayStep395 PathwayStep398 PathwayStep383 PathwayStep387 Ribosomal scanning and start codon recognition GTP hydrolysis and joining of the 60S ribosomal subunit Recycling of eIF2:GDP Formation of the ternary complex, and subsequently, the 43S complex Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S Formation of a pool of free 40S subunits Cap-dependent Translation Initiation Reactome Database ID Release 4372737 Reactome, http://www.reactome.org ReactomeREACT_2099 Translation initiation is a complex process in which the Met-tRNAi initiator, 40S, and 60S ribosomal subunits are assembled by eukaryotic initiation factors (eIFs) into an 80S ribosome at the start codon of an mRNA. The basic mechanism for this process can be described as a series of five steps: 1) formation of a pool of free 40S subunits, 2) formation of the ternary complex (Met-tRNAi/eIF2/GTP), and subsequently, the 43S complex (comprising the 40S subunit, Met-tRNAi/eIF2/GTP, eIF3 and eIF1A), 3) activation of the mRNA upon binding of the cap-binding complex eIF4F, and factors eIF4A, eIF4B and eIF4H, with subsequent binding to the 43S complex, 4) ribosomal scanning and start codon recognition, and 5) GTP hydrolysis and joining of the 60S ribosomal subunit.