Synthesis of dolichyl-phosphate mannose Post-translational modification: synthesis of GPI-anchored proteins GENE ONTOLOGYGO:0006501 Glycosylphosphatidyl inositol (GPI) acts as a membrane anchor for many cell surface proteins. GPI is synthesized in the endoplasmic reticulum. In humans, a single pathway consisting of eleven reactions appears to be responsible for the synthesis of the major GPI species involved in membrane protein anchoring.<p>As a nascent protein fated to become GPI-anchored moves into the lumen of the endoplasmic reticulum, it is attacked by a transamidase complex that cleaves it near its carboxy terminus and attaches an acylated GPI moiety. The GPI moiety is deacylated, yielding a protein-GPI conjugate that can be efficiently transported to the Golgi apparatus. Reactome Database ID Release 43163125 Reactome, http://www.reactome.org ReactomeREACT_1853 Synthesis of glycosylphosphatidylinositol (GPI) Attachment of GPI anchor to uPAR PathwayStep3097 PathwayStep3094 PathwayStep3082