Synthesis of UDP-N-acetyl-glucosamine Authored: Dall'Olio, GM, 2009-11-10 Edited: Jassal, B, 2009-11-10 GENE ONTOLOGYGO:0006048 Reactome Database ID Release 43446210 Reactome, http://www.reactome.org ReactomeREACT_22394 Reviewed: Gagneux, P, 2010-04-16 UDP-acetylglucosamine acts as a donor for the first two steps of the N-glycan precursor biosynthesis pathway, and is later used as a substrate for further modifications after the precursor has been attached to the protein. It is synthesized from fructose 6-phosphate, glutamine, acetyl-CoA, and UTP in four steps. PathwayStep3105 PathwayStep3103 PathwayStep3104 PathwayStep3102 Formation of glucosamine 6-phosphate from fructose 6-phosphate and glutamine Isomerization of GlcNAc6P to GlcNAc1P GlcNAc1P is dephosphorylated to UDP-N-acetyl-glucosamine Acetylation of glucosamine 6-phosphate to GlcNAc6P