Post-chaperonin tubulin folding pathway Alpha and beta tubulin folding intermediates are formed through ATP–dependent interaction with TriC/CCT. In order to form a functional heterodimer, these folding intermediates undergo a series of interactions with five proteins: (cofactors A-E) following release from TriC/CCT (reviewed in Cowan and Lewis et al., 2001). These interactions are described in the reactions below. Ultimately, alpha tubulin, when associated with cofactor E, interacts with cofactor D-bound beta-tubulin. The entry of cofactor C into this complex results in the discharge of native heterodimer triggered by GTP hydrolysis in beta tubulin (Tian et al., 1997). Authored: Matthews, L, 2008-12-01 04:46:41 Edited: Matthews, L, 2009-02-21 04:38:35 GENE ONTOLOGYGO:0007023 Pubmed11868281 Pubmed17709011 Pubmed9265649 Reactome Database ID Release 43389977 Reactome, http://www.reactome.org ReactomeREACT_16967 Reviewed: Cowan, NJ, 2009-01-21 16:47:24 PathwayStep3035 PathwayStep3036 PathwayStep3033 PathwayStep3034 PathwayStep3040 PathwayStep3041 PathwayStep3038 PathwayStep3037 PathwayStep3039 Beta-tubulin:GTP + Cofactor A -> Beta-tubulin:GTP: Cofactor A alpha-tubulin:GTP + Cofactor B -> alpha-tubulin:GTP: Cofactor B alpha-tubulin:GTP + Cofactor E -> alpha-tubulin:GTP:Cofactor E Beta-tubulin:GTP + Cofactor D -> Beta-tubulin:GTP: Cofactor D Beta-tubulin:GTP: Cofactor A+ Cofactor D -> Beta-tubulin:GTP:Cofactor D + Cofactor A alpha-tubulin:GTP:Cofactor B +Cofactor E -> alpha-tubulin:GTP: Cofactor E +Cofactor B Beta-tubulin:GTP:Cofactor D:alpha-tubulin:GTP:Cofactor E:Cofactor C-> Beta-tubulin:GDP :alpha-tubulin:GTP heterodimer +Cofactor E+ Cofactor D+ Cofactor C+ Pi Beta-tubulin:GTP:Cofactor D:alpha-tubulin:GTP:Cofactor E+ Cofactor C-> Beta-tubulin:GTP:Cofactor D:alpha-tubulin:GTP:Cofactor E:Cofactor C Beta-tubulin:GTP:Cofactor D+alpha-tubulin:GTP:Cofactor E-> Beta-tubulin:GTP:Cofactor D:alpha-tubulin:GTP:Cofactor E