Vamp Lysosome Cargo Lysosome Cargo AP-1 Complex Lysosome Cargo:AP-1:Beta-arrestin:Clathrin Triskelion:Vamp Complex Clathrin Triskelion HSC70:Auxillin Complex trans-Golgi Network Derived Lysosomal Vesicle Uncoating Authored: Gillespie, ME, 2009-08-27 Edited: Gillespie, ME, 2008-05-21 20:31:41 Pubmed11994746 Pubmed16155256 Pubmed17116749 Pubmed8374173 Pubmed8524399 Reactome Database ID Release 43432688 Reactome, http://www.reactome.org ReactomeREACT_19206 Reviewed: Simpson, JC, 2009-08-27 The heat shock protein Hsc70 and auxilin, a J-domain containing protein, are responsible for clathrin disassembly through an ATP-dependent reaction. This uncoating step may be a point in the pathway subject to regulation. This final step releases the vesicle from the clathrin cage. The vesicle still contatins a specific Vamp molecule, part of the targeting and fusion mechanism that delivers the vesicle to its ultimate destination. This vesicle also contains its cargo, membrane proteins embeded in the lysosome membrane. has a Stoichiometric coefficient of 48 has a Stoichiometric coefficient of 52