Vamp Golgi-associated Vesicle Cargo Golgi-associated Vesicle Cargo AP-1 Complex Clathrin Triskelion HSC70:Auxillin Complex Cargo:AP-1:Beta-arrestin:Clathrin Triskelion:Vamp Complex trans-Golgi Network Derived Vesicle Uncoating Authored: Gillespie, ME, 2009-08-27 Edited: Gillespie, ME, 2008-05-21 20:31:41 Pubmed11994746 Pubmed16155256 Pubmed17116749 Pubmed8374173 Pubmed8524399 Reactome Database ID Release 43421836 Reactome, http://www.reactome.org ReactomeREACT_19124 Reviewed: Simpson, JC, 2009-08-27 The heat shock protein Hsc70 and auxilin, a J-domain containing protein, are responsible for clathrin disassembly through an ATP-dependent reaction. This uncoating step may be a point in the pathway subject to regulation. This final step releases the vesicle from the clathrin cage. The vesicle still contatins a specific Vamp molecule, part of the targeting and fusion mechanism that delivers the vesicle to its ultimate destination. This vesicle also contains its cargo, membrane proteins embeded in the Golgi-associated vesicle membrane. has a Stoichiometric coefficient of 48 has a Stoichiometric coefficient of 52