PathwayStep4646 PathwayStep4649 PathwayStep4647 PathwayStep4648 PathwayStep4653 PathwayStep4652 PathwayStep4651 PathwayStep4650 Phosphorylation of cohesin by PLK1 at centromeres CDK1 phosphorylates CDCA5 (Sororin) at centromeres PP2A-B56 dephosphorylates centromeric cohesin CDK1 phosphorylates CDCA5 (Sororin) at chromosomal arms Phosphorylation of cohesin by PLK1 at chromosomal arms Deacetylation of cohesin Resolution of sister chromatids Kinetochore assembly Resolution of Sister Chromatid Cohesion Authored: Orlic-Milacic, M, 2012-10-02 Edited: Gillespie, ME, 2012-10-05 Edited: Matthews, L, 2012-10-05 Pubmed11509732 Pubmed14730319 Pubmed15339662 Pubmed15723797 Pubmed15737063 Pubmed16541025 Pubmed17112726 Pubmed17113138 Pubmed19696148 Pubmed21111234 Pubmed21878504 Pubmed21987589 Pubmed9649503 Reactome Database ID Release 432500257 Reactome, http://www.reactome.org ReactomeREACT_150425 Reviewed: Zhang, Nenggang, 2012-10-22 The resolution of sister chromatids in mitotic prometaphase involves removal of cohesin complexes from chromosomal arms, with preservation of cohesion at centromeres (Losada et al. 1998, Hauf et al. 2001, Hauf et al. 2005). <br><br> CDK1-mediated phosphorylation of cohesin-bound CDCA5 (Sororin) at threonine T159 provides a docking site for PLK1, enabling PLK1-mediated phosphorylation of cohesin subunits STAG2 (SA2) and RAD21 (Hauf et al. 2005, Dreier et al. 2011, Zhang et al. 2011). Further phosphorylation of CDCA5 by CDK1 results in dissociation of CDCA5 from cohesin complex, which restores the activity of WAPAL in removing STAG2-phosphorylated cohesin from chromosomal arms (Hauf et al. 2005, Gandhi et al. 2006, Kueng et al. 2006, Shintomi and Hirano 2006, Nishiyama et al. 2010, Zhang et al. 2011). <br><br> <br>At centromeres, kinetochore proteins shugoshins (SGOL1 and SGOL2) enable PP2A-B56 (also a kinetochore constituent) to dephosphorylate the STAG2 subunit of centromeric cohesin. Dephosphorylation of STAG2 enables maintenance of centromeric cohesion, thus preventing separation of sister chromatids until anaphase (Salic et al. 2004, Kitajima et al. 2004, Kitajima et al. 2005, Kitajima et al. 2006).